Combined DNA and RNA sequencing for RNA editing event discovery

RNA editing has become a generic term for a wide array of post-transcriptional processes that change the mature RNA sequence relative to the corresponding encoding genomic DNA matrix. This phenomenon, which is almost limited to eukaryotes with some exceptions, is characterized by nucleotide insertion, deletion, or substitution in various types of RNAs including mRNAs, tRNAs, miRNAs, and rRNAs , and is likely to contribute to RNA diversity. Until recently, this mechanism was considered relatively rare in vertebrates, mainly restricted to brain-specific substrates and repetitive regions of the genome, and limited to extensively validated ADAR-mediated adenosine to inosine (A-to-I) substitutions and APOBEC-mediated cytosine to uracil (C-to-U) changes.

In silico in-depth analyses of transcriptomic data with UNIX

To support molecular and cell biologists in their quest for autonomy when dealing with bio-informatics, bio-statistics, genomics or systems biology, Pr Sandrine Lagarrigue - supported by Agrocampus Ouest - started offering a highly multidisciplinary and modular workshop dedicated to biologists ten years ago. I joined the teaching staf in 2014 and organize since then 4 yearly training days on sequence bio-informatics and statistical analyses for genomic and epigenomic data. The workshop dedicated to RNA-seq analysis address the following topics: